Recognition of CD1d-sulfatide mediated by a type II natural killer T cell antigen receptor

O Patel; DG Pellicci; S Gras; ML Sandoval-Romero; AP Uldrich; T Mallevaey; AJ Clarke; J Le Nours; A Theodossis; SL Cardell; L Gapin; DI Godfrey; J Rossjohn

Journal article

Recognition of CD1d-sulfatide mediated by a type II natural killer T cell antigen receptor

O Patel, DG Pellicci, S Gras, ML Sandoval-Romero, AP Uldrich, T Mallevaey, AJ Clarke, J Le Nours, A Theodossis, SL Cardell, L Gapin, DI Godfrey, J Rossjohn

Nature Immunology | NATURE PUBLISHING GROUP | Published : 2012

DOI: 10.1038/ni.2372

Abstract

Natural killer T cells (NKT cells) are divided into type I and type II subsets on the basis of differences in their T cell antigen receptor (TCR) repertoire and CD1d-antigen specificity. Although the mode by which type I NKT cell TCRs recognize CD1d-antigen has been established, how type II NKT cell TCRs engage CD1d-antigen is unknown. Here we provide a basis for how a type II NKT cell TCR, XV19, recognized CD1d-sulfatide. The XV19 TCR bound orthogonally above the A' pocket of CD1d, in contrast to the parallel docking of type I NKT cell TCRs over the F' pocket of CD1d. At the XV19 TCR-CD1d-sulfatide interface, the TCRα and TCRβ chains sat centrally on CD1d, where the malleable CDR3 loops dom..

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University of Melbourne Researchers

Daniel Pellicci Author

Adam Uldrich Author

Dale Godfrey Author

Jamie Rossjohn Author

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Awarded by National Institutes of Health

Funding Acknowledgements

We thank the staff at the MX2 beamline of the Australian synchrotron for assistance with data collection and Monash Macromolecular Crystallisation Facility for crystallization experiments. Supported by the National Health and Medical Research Council of Australia (D.I.G. and J.R.), the Australian Research Council, the Cancer Council of Victoria, the US National Institutes of Health (AI090450 and AI092108 to L.G.), the Swedish Research Council (S.L.C.), the Swedish Cancer Society (S.L.C.) and Monash University (S.C. and J.R.).